Composite

Part:BBa_K531009:Design

Designed by: Alexander Aaring   Group: iGEM11_Grinnell   (2011-09-23)


PrsaA + Caulobacter optimized dspB + rsaA C-term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1064
    Illegal NgoMIV site found at 1366
    Illegal AgeI site found at 1489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 865


Design Notes

Stop codon at the end of dspB removed to ensure expression of the rsaA C-terminal.


Source

PrsaA</i> and rsaA C-terminal secretion tag both come from the genome of Caulobacter lab strain CB15N. IDT synthesized dspB so as to codon optimize it for expression in Caulobacter.

===References===